The question that this text will endeavor to answer is that if the essence of our total human biological constructs are operating in a “Quantum State of Uncertainty”, how best can medical science find therapies to treat their pathological problems.
Why should there be any conjecture that our present day human biological essence constructs are operating in a quantum state? Recent research has shown that in a vast variety of life forms performing photosynthesis, which has been deemed a quantum mechanical activity, and is a vital aspect of their existence. (1Ball) These species include primitive bacteria, all types of algae, and all modern plants. Migrating birds utilize photon-electron entanglement to sense the Earth’s magnetic fields to navigate their direction. (2 Birds). There is a possibility that all animals that use sonar sensing activities may also be utilizing a form of quantum state energy. All elements have electrons orbiting around their proton-neutron nuclei; the number of electrons in their orbital peripheries correspond to the number of protons. Connectivities of all molecules, cells, tissues and organs are dependent upon electrons.
Our universe came into existence in a quantum state of uncertainty. The subatomic particles that became manifested matter, quarks and electrons, still display at their essence levels enigmatic behaviors, especially electrons. Though electrons made our modern world possible, its unpredictable bizarre activity at the subatomic level still is a mystery to the most learned physicists.
There is still a total lack of understanding of the orbiting (called orbitals) of electrons around simple elements and/or combinations of stable molecules.
Why would the information in the previous paragraphs have anything to do with human therapeutics? The answer to that question is that one cannot disregard the quantum mechanical initiation of our present universe and its evolutionary continuum. The original quarks and electrons that became manifested in our the universe’s initiation are still here and are still operating in the way they were operating 13 billion years ago. They are the same basic evolutionary contructs that makeup our human bodies.
One can compartmentalize human bodies as diverse molecules that make up skin, brain tissue, lung, liver, etc., their essences are still the basic units of quarks and electrons. One can further compartmentalized the activities of human tissues as biological, biochemical, physiological etc., the essence activity still starts with the basic aspects of quarks and electrons. Brain activity and the mysterious phenomenon that is called consciousness, still operates within and through the essences of the basic constructs of quarks and electrons.
The failure of medical research to take into account that the evolutionary continuum of quantum mechanics is still in operation in the essence of the most modern human bodies has negated all efforts to find therapeutic agents. This text will endeavor make suggestions to overcome this failure.
It has show that primitive bacteria were present in abundance 3.5 billion years ago (3) From these humble beginnings, modern multicellular organisms came into existence. The highly evolved complex differentiated cells that create their specific tissues and organs may hold the clue of how exacting molecules may be obtained.
THE FOLLOWING SUGGESTIONS WILL CONTAIN QUESTIONS OF ETHICAL AND MORAL CONSIDERATIONS IN OBTAINING THE CELLS FOR CELL CULTURING AND THE PURIFICATION PROCESSES.
The DNA of all differentiated human cells initiate the production of very specific molecules that are relevant that that exact cell type.
PAGE analysis of Proteins from cells
Two Lanes on left are from one call type (human foreskin fibroblasts)
Two Lanes on right are from another cell type (MRC-5 cells; human fetal lung fibroblasts)
Each Protein forms a horizontal band with the largest proteins on top
These specific specialized molecules for each of these differentiated cells have their specific activity in orchestrated cooperation with all other molecules of that particular cell. The molecular complexity within each of these live cells is overwhelmingly impossible to grasp. The cells shown below are fetal lung fibroblasts showing different areas of the same cell magnified 37,500 times. These stereo-image photographs show a remarkable 3-dimensional complexity when viewed with 3-d lenses. (J&P )
It is important to be aware that the above picture only represents the detail of the dead cell, it is known that million of reaction are actively taking place in the live cell every few seconds.
The question now is what is relevant about the above information?
The answer is, that growing differentiated cell cultures with specialized technologies can produce molecules that are specific for that particular cell type. The technology to be used is culturing the specific cell type in roller bottles on slowly revolving roller apparatus in a warm room at 37 degrees centigrade (seen below).
Using sterile microbiology techniques the normal human cells attach themselves to the periphery of the inside of the roller bottle A special growth media is used to expand the culture until it becomes completely confluent. The growth media is then discarded, and replaced with a special production media. Normal human cells cover the entire inside of the roller bottle in a single layer. The single layer is of extreme importance.
In all normal differentiated cells, tissues, and organs, there is a continuos connectivity of all those entities. This connectivity is a major part of the information network in order to keep the homeostasis of each differentiated cell, tissue, and organs. It is an enigmatic fed-back system to the cells DNA, which determines which molecules that are aging, being destroyed, or wearing out, and needs replacing. This is the exact mechanism wherein the cells will send healing molecules to replace pathological ones. This is the way our bodies respond to all intrusions.
In these roller bottles that are just a single layer, an unusual phenomenon occurs. The cells are confluent, attached to each other and also attached to internal wall of the roller bottle, however, they have their vast surface areas exposed to the production media. The cells have dramatic reaction by not having their similar cells at their exposed surfaces. They are therefore induced to believe that they are wounded at the exposed surfaces. They start to produce their wound healing molecules, sending them to their surfaces. These molecules end up in the production media. These molecules are unique for every different cell type.
Harvesting the production media and adding fresh production media initiates the purification protocols for obtaining the normal human molecules that can be used therapeutically in many diverse clinical applications. Depending upon the cell type, the simple harvesting and adding fresh production media can continue for 1 to 4 months. There are several simple assays that can be used to determine the protein production of the harvested production media. Also depending on each type of cell culture, a decision will be made whether to start a new culture, or in many instances an expansion of the original culture in new roller bottles will produce good yields. Nonetheless, all of these normal cell cultures do exhibit a propensity to become senescent eventually. Again, assays of protein production can allow a proper judgement.
Herein is a revelation about the precise way the cells produce their healing molecules. Although there a myriad of diverse molecules that are produced and sent though the cell’s membrane, they are “linked” together. A single pass through the proper separation media will allow the molecules to still be linked. A pass through a gentle sterilizing filter, and a further determination as to the preferred dosage will be utilized before lyophilization. These freeze-dried molecules could have a 2 year shelf life. Their reconstitution before clinical application will prove be the most innovative natural normal therapeutic agents ever produced.
At this point in this text we will address the significance of the molecules that are made by our cells to heal themselves and have a substantial participation in maintaining cellular, tissue, and organ homeostasis. These genetically generated molecules for the most part travel from the cell’s interior to the exterior of the cell. They reside in specific complex arrangements depending upon the cell type. These outside molecular complexes are named the Extracellular Matrix, designated the EMC. The EMC complex is further referred to as the cell’s “Microenvironment”.
The EMC’s microenvironment consist of structural and multifunctional molecules. The EMC relays both biochemical and biophysical cues to and from the cells to modulate cell behavior and function. (7) A variety EMC molecules have shown to attach to various cell surface receptors called Integrins; 21 have been found. They are combinations of two types of transmembrane proteins (called heterodimers) that intimately link the ECM on outside of the cells to the actin cytoskeleton ( a molecule responsible for cell shape) to the inside of the cell. Relevant premises presented by a research group are as follows:
1. The EMC and the cell are to be viewed as a single functional unit; that is , there exists a “dynamic reciprocity” between the cell and its ECM. A change in one will affect the other. A disruption in proper interactions could lead to abnormal function and hence disease and possibly cancer.
2. Transmembrane proteins (integrins and others) are involved in signal transmission between the EMC molecules and elements that regulate GENE expression.
3. Cell-ECM interactions not only affect the cytoskeleton and cellular organization but also influence the way that cells interact with one another as well as the overall three-dimensional integrity of the tissues as a whole
From the preceding, clinically applying tissue specific EMC molecules as therapeutic agents to pathological areas wherein a EMC disruption exists at the least makes sense from a research point of view. Normal human molecules have never shown any adverse effects when applied clinically. Examples of their clinical application are demonstrated by the FDA approved products for several types of chronic wound healing, Apligraf and Dermagragh. Both of these products use live fibroblasts attached to a barrier to cover chronic wounds. The live cells themselves do not become incorporated into the tissues, or else an immunological event would occur. Nonetheless, the wounds using these therapies heal better than other methodologies. This is due to the EMC molecules from the live cells float into the chronic tissue greatly enhancing their healing rate. To date, there have been very minimal side effects.
The therapies proposed herein constitute Transplantation of Tissue Specific “Normal Human Molecules” to areas of bodies that have had a disruption in the dynamic reciprocity between the Cell-EMC. Medically called pathology. BODIES WILL RECOGNIZE THESE THERAPIES AS SELF, BECAUSE THEY ARE SELF.
At this time in this text we shall return to the original question of how the dynamic feed-back from the ECM microenvironment corresponds to and affects the cell’s genetic machinery. Recent research in the newly explored field of “Quantum Biology” has shown irrefutably that electrons operate in a coherent fashion in primitive single cell organisms as well as modern multicellular plants. Primitive 3 billion year old bacteria are known to have utilized photosynthesis in their every day function. These processes involve ELECTRONS. Recent experiment have shown that electrons can be active at spectacularly cold temperatures,(9) at ambient (room temperature)(10) or in species residing in high temperatures. (11) From a evolutionary prospective electrons which have been in existence since the the beginning of this universe, fit nicely into its activities of live species for billions of years. There has always been the speculation of how information flows from the outside of the cell, its microenvironment, to the cell’s inside? The answer; its the coherent electron. Nature would not need a new invention for a system that was in existence for at least 3 billion years.
Each particular ECM contains many types of molecules dependent upon the cell type. When reconstituted, these lyophilized therapeutic agents will prove to have the same bioactivity as the original molecules; WITH ALL OF THEIR ELECTRONS!
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